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Clinical signs of the disease were considered when animals had rectal temperatures above 40C and hematocrit below 60% of the basal value of each calf

It is, nevertheless, noteworthy to stress that morphea does not transit to SSc

Maternal prenatal folate supplementation induced aberrant DNA methylation of adipose triglyceride lipase (ATGL) in the liver and LPL in white adipose tissue, resulting in a significant decrease in the expression of ATGL and LPL, exacerbating the adverse effects of HFD on lipid metabolism in their offspring (109)

3T3-L1 cells were cultured in 60 mm diameter dishes (8.4 104 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)