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To evaluate NRF2-dependent regulation of these genes, we generated two KEAP1 -mutant NSCLC cell lines (A549 and NCI-H460) stably integrated with a doxycycline (dox)-inducible shRNA targeting NRF2

the buffer solution already contains the maleimide, in particular at a concentration of at least 0.0001 mM, preferably at least 0.001 mM, more preferably at least 0.01 mM, even more preferably at least 0.1 mM, yet even more preferably at least 1 mM or even at least 2 mM (and preferably not more than 50 mM or not more than 25 mM or not more than 10 mM)