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Library preparation For each sample, up to 200 ng of cDNA (as determined by PicoGreen quantification) was fragmented using a Covaris E220 Focused Ultrasonicator (Covaris) with the following parameters: 200 peak incident power, 25% duty cycle, 50 cycles per burst, and 10 s duration repeated 120 times

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Below are detailed insights into its research context and findings