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On this basis, more precise new-generation technologies such as high-fidelity Cas9 variants, optimized sgRNA design, base editing (Base Editing) or prime editing (Prime Editing) can be used to significantly reduce this risk

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Proteins were then mixed with SDS-PAGE loading buffer (Servicebio, China), heated at 95 C for 10 min, and 20 g protein was subjected to SDS-PAGE

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