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Description

F.GrachevaE

doi: 10.1111/j.1432-1033.1984.tb07943.x

In the presence of individual cytokinins and their combinations (BAP/Kn), no morphogenic response was observed and the explants turned brown within 3 weeks

After 24 h, the culture medium was replaced with complete medium and 0.0250.250 ml of Lenti-X 293 T supernatant containing lentiviral particles and 4 g/ml of polybrene (hexadimethrine bromide, Sigma-Aldrich, Milan, Italy), per well