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These reactive aldehydes further disrupt mitochondrial function, activate pro-inflammatory pathways (e.g., NF-B), and sensitize nociceptive neurons, contributing to neuroinflammation, neuronal dysfunction in FM ( Mitochondrial dysfunction in FM is evident from reduced electron transport chain (ETC) activity, lower oxygen consumption in muscle biopsies, and diminished bioenergetic reserve ( Neuronal and microglial mitochondrial dysfunction in FM impairs electron transport chain (ETC) activity, leading to electron leakage and superoxide (O ) formation, which contribute to ATP deficits

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Here, we demonstrated that hCTPS1 also forms filaments in solutions containing the enzymatic products CTP, ADP, and Glu (Figure 2B), indicating that product binding can induce filament assembly

The nematodes were incubated at 16C