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, immediately after anastomosis creation) or to reverse already formed thrombus at the site of anastomosis (at the 24 h after surgery)

Slides were counterstained with DAPI and coverslipped with Prolong TM Gold Antifade mountant (Thermo Fisher Scientific)

Similarly, alkaline hydrolysis of the candidate octadepsipeptide ( m/z 837.4806), followed by UPLC-TOF MS analysis, also revealed D- O -Leu-D-Ala ( m/z 202.1073, [M-H] ) and L- O -Val-L-Val ( m/z 216.1238, [M-H] ) as cleavage peptides as expected to be released from (D- O -Leu-D-Ala-L- O -Val-L-Val) 2 -OEt (data not shown)

Assuming the bands appear at the expected positions corresponding to their theoretical molecular weights, this indicates that the fusion proteins were expressed at the correct size and were not significantly degraded