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Dermatol 24 (2), 155157

More recent studies report the skin benefits of resveratrol which include anti-inflammatory, antioxidant properties that protect against UV radiation, oxidative stress by Nrf2 activation by reducing the expression of activator protein 1 (AP-1) and NF-kB factors, proliferation of fibroblasts to increase collagen (types I, II, and III), inhibition of melanogenesis, and activation of sirtuin 1 (SIRT 1, the anti-aging factor) [49, 81,82,83]

Kimmel PLSS, Patel

At 8 DIV, growth medium was replaced with defined serum-free culture medium consisting of a mixture of a buffered saline solution (10 mM HEPES, pH 7.4, 114 mM NaCl, 26.1 mM NaHCO 3 , 5.3 mM KCl, 1 mM MgCl 2 , 2 mM CaCl 2 , 5 mM glucose, 1 mM glycine, 0.5 mM C 3 H 3 NaO 3 , and 0.001% phenol red) and phosphate-free Eagle's minimum essential medium (9:1 v/v) supplemented with insulin (7.5 g/mL), transferrin (7.5 g/mL), and sodium selenite (7.5 ng/mL) (ITS supplement, Merck Millipore, #I3146)