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Dead cells were labelled by transcranially applying propidium iodide (red) 30 min before fixation

The death of dopamine-producing neurons in the substantia nigra causes Parkinsons disease.[ref] MPTP was discovered as a contaminant in illegal drugs that caused immediate and irreversible Parkinsons symptoms

21505034), Scientific Research Nursery Project of Henan University of Chinese Medicine (MP2020-23, MP2020-107), and Henan College Student Innovation and Entrepreneurship Program of Henan University of Chinese Medicine (202110471007)

The precipitate was washed with the same weight of 10% TCA three times, and the protein-bound form of glutathione was extracted with the same weight of 10% TCA-4% 2-mercaptoethanol as described above