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The optical density value of a sample was determined at a wavelength of 450 nm using an Infinite M200 PRO plate reader (Tecan, Austria)

Where indicated, the protein synthesis inhibitor cycloheximide (CHX) (Sigma-Aldrich) and the proteasome inhibitor MG132 (Sigma-Aldrich) were added to HEK293 cells cultured with a final concentration of 25 g/ml and 10 M respectively in a time-course experiment

This peptide combination enhances lean muscle mass, aids in fat loss, and promotes overall rejuvenation by stimulating growth hormone production