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2 Antioxidant activity As a potent redox regulator, melatonin (MLT) exhibits dual antioxidant mechanisms: executing direct ROS/RNS neutralization while concurrently upregulating endogenous antioxidant enzymatic systems through catalytic proficiency modulation, and its main antioxidant effect is the formation of N-acetyl-5-methoxytryptophan (AMK), deformed from the metabolite of melatonin, N1-acetyl-N2-formyl-5-methoxykynurenine (AFMK) (Galano et al., 2013)

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