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L., Lin, C

The current state of glioma data registries

After observing leakiness of the GFP from the ER to the Golgi at the steady state, due to the presence of free biotin coming from DMEM/F-12 and B27-Supplement, we sequestered it by supplying 0.1 mg/ml of NeutrAvidin Protein (Thermo Scientific, #31000) to the WiBi media for 2-3 days before performing live imaging