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Artificial optimized 5 untranslated regions (5 UTRs) including the ribosome binding sites (RBS) were generated for each gene and vector combination with the online RBS calculator of the Salis Laboratory of Penn State University 2 and were introduced via the oligonucleotides during cloning

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The NO-synthesizing capacity of mtNOS is higher than all of the cytoplasmic NOS isoforms combined (Brookes, Figure 3 Potential therapeutic targets affecting mitochondrial dysfunction and the nitric oxide pathway in metabolic syndrome Different research groups have studied various ways to regulate NO signaling pathways to modulate mitochondrial dysfunction