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Samples were then precipitated by ice cold H 2 O/MeOH/CHCl 3 (v/v 4:4:1) washed and resuspended in 50 mM HEPES buffer (pH 7.4)

KRAS G12 mutations account for approximately 65% of all KRAS mutations, with KRAS G12D accounting for 28%, KRAS G12V for 20%, and KRAS G12C for 7% [12]