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Joo Barata from the Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisbon, Portugal

Laurent Salphati View on Scopus Drug Metabolism Reviews, ISSN: 03602532 Shuai Wang

The CBS OPT and L2HGDH OPT were then constructed in pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro (#73037, Addgene), with the Nluc-P2A-coGFP genes removed

The activated NEDD8 is then transferred via a translational reaction to an E2 conjugating enzyme (UBE2M/UBC12 or UBE2F), forming a NEDD8E2 thioester complex