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doi: 10.3390/ijms22179459 107 ZulloAHassanCCristofariFAndrianiADe FrancescoVIerardiEet al

Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation

gametophytic control) showed SOD levels comparable to induction stages

Cells were treated with PEMS buffer containing 1% Triton X-100 at room temperature for 1 min, washed twice with PEM buffer, and incubated in PEMBAL buffer (100 mM PIPES pH 6.9, 1 mM MgCl 2 , 1 mM EGTA, 1 % BSA, and 0.1 M L-lysine) at room temperature for 1 h with gentle rotation