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By contrast, inhibition of VSM- Grk2 by overexpression of the C-terminal portion of Grk2 or VSM-specific ablation of Grk2 protein expression in mice has no effect on the increased blood pressure in the two-kidney, one-clip (2K1C) model, which may be due to a balance between the increased -AR-mediated dilation and the enhancement of 1D -AR-stimulated vasoconstriction [39]