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Exercise capacity was determined by a single maximal exercise test to voluntary fatigue on a motorized treadmill (Exer 3/6, Columbus Instruments) in females [Young ( n = 10), Old Con ( n = 17), and Old mAb ( n = 22)] and males [Young ( n = 10), Old Con ( n = 9), and Old mAb ( n = 9)]

Then close the bottle tightly

Gel lanes were cut in several pieces, and proteins were reduced, alkylated, and digested by overnight incubation with trypsin (bovine sequencing grade, Roche Applied Science, Monza, IT) according to published protocols ( 2.10 Identification of selected antigen by LC-MALDI Protein immunopurifications were precipitated in ice-cold acetone overnight and resuspended in 40 L of NH 4 HCO 3 50 mM, urea 2 M solution, and digested with 5 L of trypsin solution (0.2 g/L) overnight at 37C

20 No recommendations can be made for the anticonvulsants valproate (Depakote) 19 and carbamazepine (Tegretol) 20 because of limited data