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Consistently, SureQuantbased targeted mass spectrometry using two unique CEST2.2 peptides (LSTGTIEGR and HGEDFAYAFGTNR) revealed that our transgene could also restore CEST2.2 protein levels when introduced in gas1(fc21) mutants (Fig 1L and Appendix Table S2)

J Clin Oncol 1994

Von Frey test We assessed mechanical pain sensitivity by observing the paw withdrawal reaction to von Frey filament stimulation

In respect to vesicular release, it appears unlikely that filling of vesicles with ATP by VNUT is regulated by [ATP] c (at least for the physiological concentration range), given that VNUT is an active transporter and [ATP] c is rather high