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The precipitate was extracted using 100 L of 5% trichloroacetic containing 2% 2-mercaptoethanol

However, in a dose-dependent manner, its combination with acrylamide led to the significant elevation of the number of ovarian follicles and the levels of FSH, E2 and progesterone (Reference Naimi, Shariati and Naimi31)

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If the peptide were made in the body, Szabo and his colleagues wrote, one would expect that over the course of decades, researchers would have identified the gene that encodes it, the cells that make it, the receptors that it interacts with, and the levels at which it occurs in the body