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3T3-L1 cells were cultured in 60 mm diameter dishes (8.4 104 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)

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These studies often took place for a few week- or month-long period, making it difficult to understand the longer-term potential of sublingual B12 at maintaining serum B12 levels