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Vitamin B 12 serum analysis Mouse serum was diluted at a 1:20 ratio in PBS and holotranscobalamin (holoTC) was measured using an ADVIA Centuar Immunoassay System (SIEMENS) with ADVIA Centuar Vitamin B 12 Test Packs (07847260) according to the manufacturers instructions

[7] Physicians therefore evaluate each patients health status before recommending NAD+ injections and exercise caution in certain scenarios even in the absence of formal contraindication labels