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First, 3-phosphoglycerate is oxidized to 3-phosphohydroxypyruvate with concomitant production of NADH, catalyzed by phosphoglycerate dehydrogenase (PHGDH)

Once reconstituted, the vial lives in the refrigerator, never the freezer, since freezing a mixed solution can damage the peptide

This worsens electron leakage and ROS production, triggers apoptosis and inflammation, and accelerates hepatocyte dysfunction and fibrosis in CRHM syndrome