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PLoS Biol 21:e3001983 Quinlan CL, Perevoshchikova IV, Hey-Mogensen M, Orr AL, Brand MD (2013) Sites of reactive oxygen species generation by mitochondria oxidizing different substrates

As diamide is a mild oxidizing agent compared to H 2 O 2, less dimers were observed in this case, as shown by electrophoresis on a non-denaturing PA gel ( Figure 3A )