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Description

J Trace Elem Med Biol 52:37

Mengfan et al

In reaction mixture without enzyme solution, varying amounts of glutathione (0-300 l of stock solution giving 0-260 M final glutathione concentrations) were reacted with 150 l of monobromobimane solution (final concentration 50 M) in presence of phosphate buffer (3.0 ml) in cuvettes

On the cytoplasmic side, Threonine 40, conserved in MGST1, was the closest residue with 4.4 from the threonine oxygen to one of the fatty acid carboxyl oxygens