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The expression of ERK, p38 MAPK, and AP-1 can increase transcriptional activity within the NF-kB pathway, consequently triggering skin inflammation and accelerating the process of skin aging (87)

They were cloned into pKO3, and following the published protocol (Link et al ., 1997), this vector was used to sequentially select and then screen for replacement of the wildtype trxC allele with the deletion allele