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Acta Physiol Plant 31:393400 Soltani Y, Saffari VR, Moud AAM, Mehrabani M (2012) Effect of foliar application of -tocopherol and pyridoxine on vegetative growth, flowering, and some biochemical constituents of Calendula officinalis L

The tissues were then rinsed and incubated with a biotinylated secondary antibody (1:500, Cat# GB23303, Servicebio, China) and streptavidin-HRP (Cat#SP-9001, ZSGB-BIO, China)

Lowering homocysteine supports methylation, serotonin production, and long-term brain health

To quantitatively measure the expression levels of the 60 genes extracted through microarray analysis, RT-qPCR was used