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Minimal Automation: Operators relied heavily on manual oversight, which left room for human error and inconsistent performance

Nat Genet 41:946952 Lee EC, Yu D, Martinez de Velasco J, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG (2001) A highly efficient Escherichia coli -based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA

BACKGROUND: Thyroid hormones play a vital role in regulating our body's metabolism

Active cancer is a relative contraindication: because GHK-Cu promotes cell growth, providers avoid it in uncontrolled malignancy (though ironically it also has anti-cancer gene effects)