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Reduction of tetrazolium dye MTT [3-(4-5 dimethyl thiazol 2yl) 2, 5-diphenyl tetrazoium bromide] to its formazan was generated by pyrogalol auto- oxidation and super oxide inhibition a n d t h e r e a c t i o n wa s i n h i b i t e d b y DMS O a n d read at 570 nm and is expressed as U/ml

Moreover, Wang et al (138) found that AS-IV pretreatment concentration-dependently suppresses -SMA, FN and collagen IV expression in MCs induced by HG and the prohibitive effect of AS-IV on the activation and proliferation of MCs induced by HG is still related to autophagy activation mediated by SIRT1-NF-B signaling transduction

We developed a computational workflow using scRNA-Seq data to assess cellular metabolism during viral infection

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