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Serum brain-derived neurotrophic factor levels in treatment-nave boys with attention-deficit/hyperactivity disorder treated with methylphenidate: an 8-week, observational pretestposttest study

To optimize SeNPs concentration for subsequent semen cryopreservation, three semen samples were individually split into four equals and diluted to a final concentration of 6.0 10 7 spermatozoa/ml with the basic extender supplemented with 0 g/ml SeNPs, 1 g/ml SeNPs, 2 g/ml SeNPs, and 4 g/ml SeNPs