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Samples were mixed with loading buffer prior to electrophoresis and loaded onto either (i) a 16% denaturing polyacrylamide gel (prepared in TBE buffer with 20% formamide), where an aliquot of the reaction mixture was mixed with an equal volume of loading buffer and electrophoresed at 500 V for 90100 min at room temperature, or (ii) a 15% Novex TBE Urea Gel (Thermo Fisher Scientific), where 1 L of the reaction mixture was mixed with 9 L of loading buffer and electrophoresed at 200 V for 8090 min