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pH:5.5 7.4

After solubilization of neutral-red stain with 0.1 mL of acidified alcohol (10% acetic acid, 40% ethanol in distilled water) the absorbance was measured in a microplate reader at 550 nm

Selenium supplementation was done by first mixing the Se-methionine (Sel-Plex, Alltech Inc., Nicholasville, KY, USA) with 100 g of the diet, then thoroughly blending it into the remaining feed to ensure uniform distribution

Given the role of NAD + in modulating the processes described above, NAD + -boosting interventions are being investigated for supporting mitochondrial homeostasis