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Pierce TM BCA protein assay kit was obtained from Thermo Scientific , WesternBright TM ECL-spray (K-12049-D50) was obtained from Advansta , GSTP1 monoclonal antibody, clone 3F2C2 and GADPH antibody goat anti-rabbit IgG H + L HRP were obtained from Merck , Amersham TM Protran TM Premium Nitrocellulose blotting membrane 0.45 m was obtained from Cytiva