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290,291 Therefore, the prevailing view is that therapies combining TGF- signaling inhibitors with other anti-tumor therapies is a promising strategy that not only inhibits advanced tumor progression but also reverses treatment resistance

Live-cell microscopy RAW 264.7 macrophages were seeded in Nunc 8-well Lab-Tek chambered coverglass (ThermoFisher) at 23 10 5 cells per well in 500 l complete minimum essential medium (MEM, Gibco) supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 C with 5% CO 2 for 16 h

Generation and interactions of reactive oxygen species Reactive oxygen species can be generated from many sources, which can be either endogenous or exogenous

Ensure adequate hydration To increase tolerance, pair it with Vitamin C, probiotics, and fiber-rich foods