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Additionally, through subcellular fractionation and Western blot analysis, we detected a significant increase in the nuclear level of PARP-1 upon SRY overexpression in HepG2-SRY cells and Sry -TG mouse hepatocytes (Fig

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The samples were subsequently incubated with 10 M mitoSOX working solution at 37 C for 30 min under light-protected conditions, followed by nuclear staining with Hoechst 33,342 solution for 5 min

While many of those results may be attributed to GSH depletion, the contribution of GCLC to -glutamyl peptide synthesis and glutamate scavenging may also play a very important role, particularly in the context of xCT inhibition, where cells cannot export glutamate