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Bacterial species associated with immunological and metabolic health were more prevalent among centenarians, including taxa from Ascomycetes, Actinobacteria, and Verrucomicrobia, as well as the genera Akkermansia, Christensenellaceae, and other Lactobacillus species (Ratanapokasatit et al., 2022

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An auto-sampler with a volume of 20 L was used

Following primary antibody incubation, cells were treated with goat anti-rabbit IgG H&L Alexa Fluor 488 (ab150077, Abcam), donkey anti-goat IgG H&L Alexa Fluor 647 (ab150131, Abcam) and DAPI (Invitrogen) for 1 hour in the dark at room temperature