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Striatum was isolated from two forebrain sections from both hemispheres under the stereotactic microscope, placed in the dissection buffer (Sucrose 300 mM, Imidazole 25 mM, EDTA 1 mM, pH 7.2 with the addition of proteinase inhibitors 0.4 g/ml leupeptin, 0.1 mg/ml pefabloc) and homogenized with for 15 s

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Sua formulao se mostrou compatvel com os princpios dessas dietas, permitindo uma ingesto baixa de carboidratos sem comprometer a energia necessria para atividades fsicas intensivas