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Most approaches focused on enhancing the supply of energetic substrates, manipulating metabolic fluxes, or optimizing mitochondrial function 16

Briefly, cell or tissue protein lysates were assayed in transparent 96-well plates (Greiner 655101) in the presence of 1 mM of the GGT substrate L-glutamic acid -p-nitroanilide (GpNA, Cayman 36209) and 20 mM of the transpeptidation acceptor glycyl-glycine (Sigma-Aldrich G1002) in Tris-HCl 100 mM pH 8

The GHK-CU dosing protocol delivers copper in a bioavailable form that supports enzymatic functions including lysyl oxidase for collagen cross-linking and superoxide dismutase for antioxidant defense

doi: 10.1042/BCJ20160510 92 TsaiM