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aureus produces alpha-toxin, a pore-forming toxin that inserts itself into the membranes of host cells, causing cell lysis and contributing to tissue destruction

The sections were then incubated in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 1% Triton-X, .05% Tween-20, and .05% sodium azide) at room temperature for 1 h and incubated at 4C overnight with the following primary antibodies