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Next, 1 M of the folded RNA was incubated in a 40 L reaction mixture containing 1.03 PKN buffer, 1 mM EDTA, and either an Rbs-VQ precursor or a VQ-free compound

2019), namely the Columbia (Col-0) ecotype of Arabidopsis thaliana (L.) as a wild type control and Atgstf8 (At2g47730) and Atgstu19 (At1g78380) T-DNA insertional lines

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Nevertheless, we found that a small fraction (~10%) of condensed, ciprofloxacin-treated cells briefly became turgid, as indicated in phase-contrast by phase-light regions becoming phase-dark and increases in cell size, so that hypoosmotic shock appeared to transiently reverse cytoplasmic condensation in these cells (Fig