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The reaction was quenched by replacing the biotin solution with ice-cold 10 mM glycine in TBS (pH 8.0)

Herrmann W, Schorr H, Obeid R, Geisel J

PMID: 1299794 Kovalzon VM (2006) Delta sleep-inducing peptide (DSIP): a still unresolved riddle

However, conditions affecting the gastrointestinal tractincluding pernicious anaemia, Crohn's disease, coeliac disease, or gastric surgerycan significantly impair absorption, necessitating alternative supplementation routes such as intramuscular hydroxocobalamin injections, which deliver B12 directly into the bloodstream