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Coverslips were stained with DAPI (1 g/ml

To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol

Part one reviews our understanding regarding adverse reactions to feline vaccination, while part two summarises the advice on how to use vaccinations to best effect, while minimising the risk of inducing disease