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Through an integrative approach combining coarse-grained molecular dynamics simulations, free energy calculations, in vitro lipid-binding assays, and subcellular analyses in embryonic mouse brains, we show that the M105I mutation conceals a critical hydrophobic loop in the N-terminal region and reduces its affinity for plasma membranederived lipids

To determine reference ranges, labs test the blood of a large representative population sample (chosen based on characteristics like age, sex, and general health status) and use statistical analysis to identify the middle 95 percent of values for the nutrient in question

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10.1021/bc700184b 11 BernsmannF.BallV.AddiegoF.PoncheA.MichelM.GracioJ