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Wang X, Shu Q, Song L, Liu Q, Qu X, Li M

Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol

Images were captured with a light microscope (Leica)

47 In conclusion, these findings mentioned show that seemingly disparate functions of GST in fact work on several aspects of tumor carcinogenesis, making it an ideal molecule for further antitumor therapy research