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Determination of intracellular cysteine The cysteine content in the cells was determined by the method of Cotgreave and Moldeus (1986) with slight modifications

Although the separation is feasible at alkaline pH, acidic pH was preferred due to improved column stability and better detector response for most analytes

Fecal Eosinophil Cationic Protein (fECP) : Levels of ECP in stool are significantly elevated in patients with active IBD compared to healthy individuals

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