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This method has its pros and cons, while being helpful to measure cytokines potentially at a more localized level, these PBMCs can be affected by cryopreservation, length of storage, and the overall processing methods

4 Mediation of comorbidity It is important to note that the mechanistic pathways described below are largely inferred from preclinical models, in vitro experiments, and observational human studies

The serine-containing group of peptides appears to be essentially cytotoxic, whilst the tyrosine-containing group has the capacity to trigger immunological reactions in CD patients [17]

doi: 10.1007/pl00000664 3 Association of Official Analytical Chemists (AOAC)