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For gene expression analysis, 1 g of total RNA for each sample was retrotranscribed into complementary DNA (cDNA) with the iScript TM cDNA Synthesis Kit (BIORAD, Hercules, CA, USA), following the manufacturer's instructions, using the GeneAmp PCR System 9700 (Perkin Elmer, Waltham, MA, USA)

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This approach helps you stay consistent with your training, which is essential for long-term muscle development and joint regeneration