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Always inject into different sites in a rotation patternabdomen, thigh, and upper arm areasto avoid tissue irritation and hardening that can increase leakage risk

Although neutralizing antibody titers are indeed associated with protection against infection (primarily because they opsonize pathogens, making it easier for macrophages to recognize them), excessive antibody production can have untoward effects such as vaccine-associated enhanced disease (VAED), potentially mediated by the phenomenon of antibody-dependent enhancement or pathogenic priming

The features of the cell lines are described in detail in the site and the references from 71, 76105, and reviewed in ref

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