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The total radical-trapping antioxidant parameter (TRAP) assay quantifies the capacity of antioxidants to delay fluorescence decay in a probe (such as R-phycoerythrin or dichlorofluorescein) under controlled peroxidative conditions initiated by AAPH-generated ROO

Eriau, E., Paillet, J., Kroemer, G

Cultural examination of liver tissue or bile is recommended, as neutrophilic cholangitis is believed to result from ascending bacterial infection from the intestine

SFN induces a pro-oxidative milieu in untransformed human T-cells ROS are ubiquitously generated and recognized as important signaling molecules for T-cell activation, but excessive ROS generation or prolonged exposure to high ROS concentrations impair T-cell functions ( 2 DCFDA and treated with SFN prior to flow cytometric analysis