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Each of these dysfunctional synaptic-related functions due to oxidatively modified key proteins is observed in AD and MCI brains, and we propose that these oxidatively modified proteins contribute to the cognitive dysfunction and related clinical observations in AD and MCI

For Plp-staining cells were washed and stained with anti-Plp 1:500 (NB100-1608) (Novous Biologicals, Littleton, CO) overnight followed by anti-chicken-Cy3 (Thermo Fisher Scientific, Waltham, MA) and co-stained with CellMask and Hoechst (Thermo Fisher Scientific, Waltham, MA) for 5 min at +4 C